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Valiant Co Ltd lsm lymphocyte separation medium
Lsm Lymphocyte Separation Medium, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 96/100, based on 1011 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lsm+lymphocyte+separation+medium/LSM+lymphocyte+separation+medium/pmc13131433-44-15-19
Average 96 stars, based on 1011 article reviews
lsm lymphocyte separation medium - by Bioz Stars, 2026-09
96/100 stars

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Isolation:

Article Title: Therapeutic nuclease compositions and methods
Article Snippet: DNA sequence analysis of positive clones was performed with ABI Ready Reaction Mix v 3.1 and analyzed using an ABI 3730 XL DNA sequencer. .. Human PBMCs were isolated from heparinized human blood diluted 1:1 in D-PBS and layered over LSM Lymphocyte Separation Medium (MP Biomedicals, Irvine, CA) Ficoll gradients. .. Mouse spleen RNA was isolated using QIAgen RNAeasy kits (Valencia, CA) from approximately 5×10 e6 splenocytes.

Article Title: Cofilin-1 is a Redox-Sensitive Guard of the NLRP3 Inflammasome
Article Snippet: BMDMs were replated in 12-well plates (1.0 × 10 6 cells per well) in DMEM (Invitrogen) containing 10% FBS and antibiotics.1 day prior to experiments. .. Human peripheral blood mononuclear cells (PBMCs) were isolated by LSM-Lymphocyte Separation Medium (50494, MP Biomedicals) from freshly drawn peripheral venous blood from patients, and plated in 12-well plates(2.0 × 10 6 cells per well) in RPMI 1640 (Invitrogen) containing 10% FBS and antibiotics. cDNAs encoding WT or various mutant cofilin-1 was cloned into pLNCX2 vector (631503,Takara) and then transfected into RetroPack PT67 cells (631510, Takara). ..

Article Title: Long noncoding RNA Nron and its functional motif modulate macrophage-mediated inflammatory response
Article Snippet: .. Blood samples (10 mL) were collected, and peripheral blood mononuclear cells (PBMCs) were isolated using LSM-Lymphocyte Separation Medium (50494X, MP Biomedicals). ..

Article Title: LRRK2-mediated NLRC4 phosphorylation differentially regulates IL-1β/IL-18 secretion
Article Snippet: Alternatively, cells were stimulated with Salmonella typhimurium (ATCC, Manassas, VA) added at a multiplicity of infection (MOI) of 1 for 4 hours. .. PBMC were isolated from Lymphopac apheresis specimens (NIH blood bank) or whole blood using density-gradient centrifugation with LSM Lymphocyte Separation Medium (MP Biomedicals) and were washed twice with PBS. ..

Article Title: Long noncoding RNA Nron and its functional motif modulate macrophage-mediated inflammatory response.
Article Snippet: .. Blood samples (10 mL) were collected, and peripheral blood mononuclear cells (PBMCs) were isolated using LSM-Lymphocyte Separation Medium (50494X, MP Biomedicals). ..

Mutagenesis:

Article Title: Cofilin-1 is a Redox-Sensitive Guard of the NLRP3 Inflammasome
Article Snippet: BMDMs were replated in 12-well plates (1.0 × 10 6 cells per well) in DMEM (Invitrogen) containing 10% FBS and antibiotics.1 day prior to experiments. .. Human peripheral blood mononuclear cells (PBMCs) were isolated by LSM-Lymphocyte Separation Medium (50494, MP Biomedicals) from freshly drawn peripheral venous blood from patients, and plated in 12-well plates(2.0 × 10 6 cells per well) in RPMI 1640 (Invitrogen) containing 10% FBS and antibiotics. cDNAs encoding WT or various mutant cofilin-1 was cloned into pLNCX2 vector (631503,Takara) and then transfected into RetroPack PT67 cells (631510, Takara). ..

Clone Assay:

Article Title: Cofilin-1 is a Redox-Sensitive Guard of the NLRP3 Inflammasome
Article Snippet: BMDMs were replated in 12-well plates (1.0 × 10 6 cells per well) in DMEM (Invitrogen) containing 10% FBS and antibiotics.1 day prior to experiments. .. Human peripheral blood mononuclear cells (PBMCs) were isolated by LSM-Lymphocyte Separation Medium (50494, MP Biomedicals) from freshly drawn peripheral venous blood from patients, and plated in 12-well plates(2.0 × 10 6 cells per well) in RPMI 1640 (Invitrogen) containing 10% FBS and antibiotics. cDNAs encoding WT or various mutant cofilin-1 was cloned into pLNCX2 vector (631503,Takara) and then transfected into RetroPack PT67 cells (631510, Takara). ..

Plasmid Preparation:

Article Title: Cofilin-1 is a Redox-Sensitive Guard of the NLRP3 Inflammasome
Article Snippet: BMDMs were replated in 12-well plates (1.0 × 10 6 cells per well) in DMEM (Invitrogen) containing 10% FBS and antibiotics.1 day prior to experiments. .. Human peripheral blood mononuclear cells (PBMCs) were isolated by LSM-Lymphocyte Separation Medium (50494, MP Biomedicals) from freshly drawn peripheral venous blood from patients, and plated in 12-well plates(2.0 × 10 6 cells per well) in RPMI 1640 (Invitrogen) containing 10% FBS and antibiotics. cDNAs encoding WT or various mutant cofilin-1 was cloned into pLNCX2 vector (631503,Takara) and then transfected into RetroPack PT67 cells (631510, Takara). ..

Transfection:

Article Title: Cofilin-1 is a Redox-Sensitive Guard of the NLRP3 Inflammasome
Article Snippet: BMDMs were replated in 12-well plates (1.0 × 10 6 cells per well) in DMEM (Invitrogen) containing 10% FBS and antibiotics.1 day prior to experiments. .. Human peripheral blood mononuclear cells (PBMCs) were isolated by LSM-Lymphocyte Separation Medium (50494, MP Biomedicals) from freshly drawn peripheral venous blood from patients, and plated in 12-well plates(2.0 × 10 6 cells per well) in RPMI 1640 (Invitrogen) containing 10% FBS and antibiotics. cDNAs encoding WT or various mutant cofilin-1 was cloned into pLNCX2 vector (631503,Takara) and then transfected into RetroPack PT67 cells (631510, Takara). ..

Gradient Centrifugation:

Article Title: Interleukin-27 and Interleukin-2 Cooperatively Regulate CD4⁺ T Cell Subsets and Immune Imbalance in Coronary Artery Disease
Article Snippet: .. PBMCs were separated from blood samples using LSM-Lymphocyte Separation Medium (1.0770–- 1.0800 g/mL, MP Biomedicals, Santa Ana, CA) through density gradient centrifugation. ..

Article Title: Interleukin-27 and Interleukin-2 Cooperatively Regulate CD4⁺ T Cell Subsets and Immune Imbalance in Coronary Artery Disease
Article Snippet: .. PBMCs were separated from blood samples using LSM-Lymphocyte Separation Medium (1.0770–1.0800 g/mL, MP Biomedicals, Santa Ana, CA) through density gradient centrifugation. ..

Centrifugation:

Article Title: LRRK2-mediated NLRC4 phosphorylation differentially regulates IL-1β/IL-18 secretion
Article Snippet: Alternatively, cells were stimulated with Salmonella typhimurium (ATCC, Manassas, VA) added at a multiplicity of infection (MOI) of 1 for 4 hours. .. PBMC were isolated from Lymphopac apheresis specimens (NIH blood bank) or whole blood using density-gradient centrifugation with LSM Lymphocyte Separation Medium (MP Biomedicals) and were washed twice with PBS. ..



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